²úÆ·ÖÐÐÄ
YALEPIC? ´ÅÖé·¨DNAƬ¶ÎɸѡÊÔ¼ÁºÐ
100bp~10kb¾«×¼·ÖÑ¡ | »ØÊÕÂÊ¡Ý90% | ¼æÈÝAMPure XP | ÊÖ¹¤/×Ô¶¯»¯Ë«ÊÊÅä
»ùÓÚSPRIÔÀí£¬²ÉÓó¬Ë³´ÅÐÔÄÉÃ×´ÅÖéÓëÌØÖÆ»º³åҺϵͳ£¬Í¨¹ýµ÷½Ú¼ÓÑù±ÈÀý¼´¿É´Ó»ìºÏºËËáÑù±¾Öо«×¼·ÖÀë´¿»¯100bp~10kbÄ¿±ê´óС·¶Î§ÄÚµÄDNAƬ¶Î¡£»ØÊÕÂÊ¡Ý90%£¬¿ÉÌæ´úAMPure XP½ø¿Ú´ÅÖé£¬È«ÃæÊÊÅäÊÖ¹¤²Ù×÷Óë¸ßͨÁ¿×Ô¶¯»¯¹¤×÷Õ¾¡£
- ²úÆ·ÏêÇé
- FAQ
- COA
- Ïà¹ØÎÄÏ×
²úÆ·¼ò½é
- YALEPIC??´ÅÖé·¨DNAƬ¶ÎɸѡÊÔ¼ÁºÐ£¨YALEPIC??MagSEL DNA Size Selection Kit for NGS£©ÊÇSAÊÓѶ¹ÙÍø×¨ÎªNGS¶þ´ú²âÐò½¨¿âÑз¢µÄÒ»¿îDNA´óСѡÔñÓë´¿»¯ÊÔ¼ÁºÐ¡£¸Ã²úÆ·²ÉÓþßÓжÀÌØ·ÖÀë×÷Óõij¬Ë³´ÅÐÔÄÉÃ×´ÅÖéÓëÌØÖÆ»º³åҺϵͳ£¬»ùÓÚSPRI£¨¹ÌÏà¿ÉÄæ¹Ì¶¨£©ÔÀí£¬Í¨¹ýµ÷½Ú¼ÓÑù±ÈÀý¼´¿É´Ó»ìºÏºËËáÑù±¾Öо«×¼·ÖÀë´¿»¯100bp~10kbÄ¿±ê´óС·¶Î§ÄÚµÄDNAƬ¶Î¡£ÊÔ¼ÁºÐÖ§³ÖDNAµÄÑ¡ÔñÐÔ»ØÊÕÓë·ÇÑ¡ÔñÐÔ»ØÊÕ£¬ÎÞÐè·Ó/ÂȷµÈÓлúÊÔ¼Á£¬²Ù×÷°²È«¿ì½Ý£¬´ÅÖéÇåϴЧÂʸߣ¬»ØÊÕÂÊ¡Ý90%ÇÒÖÊÁ¿Îȶ¨¿É¿¿£¬ÄÜÓÐЧȥ³ýdNTP¡¢ÑÎÀë×Ó¡¢ÒýÎï¶þ¾ÛÌåµÈÔÓÖÊ£¬´¿»¯²úÎï¿ÉÖ±½ÓÓÃÓÚPCRÀ©Ôö¡¢¶þ´ú²âÐò£¨NGS£©µÈÏÂÓÎʵÑé¡£·ÖÑ¡ÔÀí¡¢²Ù×÷±ÈÀýÓëÆ¬¶ÎÉ¸Ñ¡Çø¼äÓëAMPure XPÒ»Ö£¬¿ÉÈ«ÃæÌæ´ú½ø¿Ú´ÅÖ飬´ó·ù½µµÍNGS½¨¿âÊÔ¼Á²É¹º³É±¾¡£ÌرðÊÊÅä¸ßͨÁ¿×Ô¶¯»¯¹¤×÷Õ¾£¬Âú×ã´óÅúÁ¿Ñù±¾´¦ÀíÐèÇó£¬Ò²ÊÊÓÃÓÚÆÕͨPCR²úÎï´¿»¯¡¢Ã¸ÇÐDNA»ØÊÕ¡¢ÖÊÁ£´¿»¯µÈ·Ö×ÓÉúÎïѧʵÑé¡£2~8¡æ±£´æÓëÔËÊ䣬ÑϽûÀä¶³¡£SAÊÓѶ¹ÙÍøYALEPIC?Æ·ÅÆ£¬¹ú¼Ò¸ßм¼ÊõÆóÒµ¡£
²úÆ·ÓÅÊÆ
| ÓÅÊÆÎ¬¶È | YALEPIC? MagSELºËÐıíÏÖ | ÐÐÒµ³£¹æË®Æ½²Î¿¼ |
|---|---|---|
| Ƭ¶Îɸѡ·¶Î§ | 100bp~10kbÈ«¸²¸Ç£¬Í¨¹ý±ÈÀýµ÷½Ú¾«×¼¿ØÖÆÉ¸Ñ¡ãÐÖµ | Ö÷Á÷²úÆ·¸²¸Ç100bp~1kb»ò200bp~800bp |
| »ØÊÕЧÂÊ | ¡Ý90%£¨¾Ñϸñ¹¦ÄÜÑéÖ¤£© | ÐÐÒµ±ê¸Ë80%~90% |
| ²Ù×÷°²È«ÐÔ | ÎÞÐè·Ó/ÂȷµÈÓлúÊÔ¼Á£¬´¿ÎïÀí·ÖÀë | ²¿·ÖµÍ¶Ë²úÆ·ÈÔ´æÔÚÓлúÊÔ¼Á·çÏÕ |
| ×Ô¶¯»¯ÊÊÅä | רΪÊÖ¹¤¼°×Ô¶¯»¯¹¤×÷վ˫³¡¾°ÓÅ»¯ | ²¿·Ö²úÆ·½öÏÞÓÚÊÖ¹¤²Ù×÷ |
| ´ÅÖéÖÊÁ¿ | ³¬Ë³´ÅÐÔÄÉÃ×´ÅÖ飬´ÅÏìӦǿ¡¢·ÖÉ¢¶È¸ß | ²¿·ÖÆ·ÅÆ´ÅÖé´æÔÚ²ÐÁô»ò³Á½µÎÊÌâ |
| ÎĿⴿ¶È | ¸ßЧȥ³ýdNTP¡¢ÑÎÀë×Ó¡¢ÒýÎï¶þ¾ÛÌåµÈÔÓÖÊ | ÆÕͨ´¿»¯Ð§¹û²Î²î²»Æë |
ÊÊÓ÷¶Î§
- 100bpÒÔÉÏDNAƬ¶ÎµÄÑ¡ÔñÐÔ»ØÊÕÓë·ÇÑ¡ÔñÐÔ»ØÊÕ
- NGSÎĿ⹹½¨ÖеÄDNAƬ¶Î´óСɸѡ£¨Size Selection£©
- PCR²úÎøÇвúÎïµÈµÄDNA´¿»¯Óë»ØÊÕ
- ¸ßͨÁ¿²âÐòÑù±¾µÄ×Ô¶¯»¯ÅúÁ¿´¦Àí
±£´æ¼°ÔËÊäÌõ¼þ
2 ~ 8¡æ ±£´æÓëÔËÊ䣬¿É¶ÌÔÝÊÒα£´æ£¬Ñϸñ±ÜÃâ·¢ÉúÀä¶³¡£
FAQ
Q1£ºÑÅÀñ MagSEL ´ÅÖéÊÔ¼ÁºÐÄÜɸѡ×îС¶àÉÙ bp µÄ DNA£¿¿ÉÒÔ·ÖÑ¡´óƬ¶Î DNA Âð£¿
A£º×îµÍÎȶ¨»ØÊÕ100bp ÒÔÉÏ DNA£¬µ÷Õû´ÅÖé±ÈÀý×î¸ß¿ÉɸѡÖÁ 10kb ´óƬ¶Î»ùÒò×é DNA£¬¼æ¹ËСƬ¶Î NGS ÎÄ¿âÓë´óƬ¶Î gDNA ´¿»¯¡£
A£º×îµÍÎȶ¨»ØÊÕ100bp ÒÔÉÏ DNA£¬µ÷Õû´ÅÖé±ÈÀý×î¸ß¿ÉɸѡÖÁ 10kb ´óƬ¶Î»ùÒò×é DNA£¬¼æ¹ËСƬ¶Î NGS ÎÄ¿âÓë´óƬ¶Î gDNA ´¿»¯¡£
Q2£º5ml ºÍ 60ml ¹æ¸ñµÄ NGS DNA ·ÖÑ¡´ÅÖéÔõôѡÐÍ£¿
A£º5ml£¨YNQ46001-01£©ÊʺϿÎÌâ×éСÊÔ¡¢ÉÙÁ¿ NGS ½¨¿âÊÖ¹¤ÊµÑ飻60ml£¨YNQ46001-03£©ÊÊÅä¸ßͨÁ¿ÊµÑéÊÒ¡¢×Ô¶¯»¯¹¤×÷Õ¾ÅúÁ¿Ñù±¾·ÖÑ¡¡£
A£º5ml£¨YNQ46001-01£©ÊʺϿÎÌâ×éСÊÔ¡¢ÉÙÁ¿ NGS ½¨¿âÊÖ¹¤ÊµÑ飻60ml£¨YNQ46001-03£©ÊÊÅä¸ßͨÁ¿ÊµÑéÊÒ¡¢×Ô¶¯»¯¹¤×÷Õ¾ÅúÁ¿Ñù±¾·ÖÑ¡¡£
Q3£ºÕâ¿î DNA ɸѡ´ÅÖé¿ÉÒÔÌæ´ú AMPure XP ½ø¿Ú´ÅÖéÂð£¿
A£º¿ÉÒÔ£¬·ÖÑ¡ÔÀí¡¢²Ù×÷±ÈÀý¡¢Æ¬¶ÎÉ¸Ñ¡Çø¼äÓë AMPure XP Ò»Ö£¬´¿»¯»ØÊÕÂÊ¶Ô±ê½ø¿Ú²úÆ·£¬´ó·ù½µµÍ NGS ½¨¿âÊÔ¼Á²É¹º³É±¾¡£
A£º¿ÉÒÔ£¬·ÖÑ¡ÔÀí¡¢²Ù×÷±ÈÀý¡¢Æ¬¶ÎÉ¸Ñ¡Çø¼äÓë AMPure XP Ò»Ö£¬´¿»¯»ØÊÕÂÊ¶Ô±ê½ø¿Ú²úÆ·£¬´ó·ù½µµÍ NGS ½¨¿âÊÔ¼Á²É¹º³É±¾¡£
Q4£ºÊÔ¼ÁºÐ´¢´æ²»Ð¡ÐÄÀä¶³½á±ùÁË£¬´ÅÖ黹ÄܼÌÐøÊ¹ÓÃÂð£¿
A£ºÑϽûÀä¶³£¬´ÅÖé½á±ù»áÆÆ»µ±íÃæÐÞÊλùÍÅ£¬É¥Ê§ DNA Îü¸½ÄÜÁ¦£¬Àä¶³ºóÊÔ¼ÁºÐÖ±½Ó±¨·Ï£¬Ðè 2~8¡æÀ䲨±£´æ¡£
A£ºÑϽûÀä¶³£¬´ÅÖé½á±ù»áÆÆ»µ±íÃæÐÞÊλùÍÅ£¬É¥Ê§ DNA Îü¸½ÄÜÁ¦£¬Àä¶³ºóÊÔ¼ÁºÐÖ±½Ó±¨·Ï£¬Ðè 2~8¡æÀ䲨±£´æ¡£
Q5£º´¿»¯ºó DNA »ØÊÕÂÊÆ«µÍÊÇʲôÔÒò£¿
A£º³£¼û 4 µã£º¢Ù´ÅÖéʹÓÃǰδÊÒÎÂÆ½ºâ£»¢Ú´ÅÖéÓëÑù±¾»ìÔȲ»³ä·Ö£»¢Û´ÅÖéÌí¼Ó±ÈÀý´íÎ󣻢ܴÅÖé¸ÉÔï¹ý¶È£¬°´ËµÃ÷ÊéÓÅ»¯²Ù×÷¼´¿ÉÌáÉý»ØÊÕÂÊ¡£
A£º³£¼û 4 µã£º¢Ù´ÅÖéʹÓÃǰδÊÒÎÂÆ½ºâ£»¢Ú´ÅÖéÓëÑù±¾»ìÔȲ»³ä·Ö£»¢Û´ÅÖéÌí¼Ó±ÈÀý´íÎ󣻢ܴÅÖé¸ÉÔï¹ý¶È£¬°´ËµÃ÷ÊéÓÅ»¯²Ù×÷¼´¿ÉÌáÉý»ØÊÕÂÊ¡£
Q6£ºMagSEL ÊÔ¼ÁºÐÄܲ»ÄÜÔÚ×Ô¶¯»¯ºËËṤ×÷Õ¾ÉÏ»úʹÓã¿
A£ºÍêÈ«ÊÊÅ䣬ÊÔ¼ÁºÐ»º³åÒºÅä·½Õë¶Ô×Ô¶¯»¯É豸ÓÅ»¯£¬Ö÷Á÷Æ·ÅÆ¸ßͨÁ¿¹¤×÷Õ¾¿ÉÖ±½ÓÉÏ»ú£¬ÎÞÐè¶îÍâµ÷ÊÔÅä·½¡£
A£ºÍêÈ«ÊÊÅ䣬ÊÔ¼ÁºÐ»º³åÒºÅä·½Õë¶Ô×Ô¶¯»¯É豸ÓÅ»¯£¬Ö÷Á÷Æ·ÅÆ¸ßͨÁ¿¹¤×÷Õ¾¿ÉÖ±½ÓÉÏ»ú£¬ÎÞÐè¶îÍâµ÷ÊÔÅä·½¡£
Q7£º·ÖÑ¡ºó²úÎﻹÓÐСƬ¶ÎÒýÎï¶þ¾ÛÌå²ÐÁôÔõô´¦Àí£¿
A£ºÊʵ±½µµÍ´ÅÖéÌí¼Ó±ÈÀý£¬²ÉÓöþÂÖ·ÖÑ¡·¨£¬¿É¸ßЧȥ³ý£¼100bp ÒýÎï¡¢½ÓÍ·¶þ¾ÛÌåÔÓÖÊ£¬ÌáÉý NGS ÎĿⴿ¶È¡£
A£ºÊʵ±½µµÍ´ÅÖéÌí¼Ó±ÈÀý£¬²ÉÓöþÂÖ·ÖÑ¡·¨£¬¿É¸ßЧȥ³ý£¼100bp ÒýÎï¡¢½ÓÍ·¶þ¾ÛÌåÔÓÖÊ£¬ÌáÉý NGS ÎĿⴿ¶È¡£
Q8£ºÊÔ¼ÁºÐÐèÒª×Ô±¸ÄÄЩÊÔ¼Á£¿ÊÇ·ñº¬·ÓÂȷ£¿
A£º½öÐè×Ô±¸ 80% ÎÞË®ÒÒ´¼£¨ÏÖÅäÏÖÓã©£¬Åä·½²»º¬·Ó¡¢ÂȷµÈÓлúÊÔ¼Á£¬²Ù×÷°²È«ÎÞ¶¾¡£
A£º½öÐè×Ô±¸ 80% ÎÞË®ÒÒ´¼£¨ÏÖÅäÏÖÓã©£¬Åä·½²»º¬·Ó¡¢ÂȷµÈÓлúÊÔ¼Á£¬²Ù×÷°²È«ÎÞ¶¾¡£
Q9£º³ýÁË NGS ½¨¿â£¬´ÅÖ黹ÄÜÓÃÔÚʲôʵÑ飿
A£º¿ÉÓÃÓÚÆÕͨ PCR ²úÎï´¿»¯¡¢Ã¸ÇÐ DNA »ØÊÕ¡¢ÖÊÁ£´¿»¯µÈ·Ö×ÓÉúÎïѧʵÑ飬ÊÇͨÓÃÐÍ DNA ´¿»¯´ÅÖéÊÔ¼ÁºÐ¡£
A£º¿ÉÓÃÓÚÆÕͨ PCR ²úÎï´¿»¯¡¢Ã¸ÇÐ DNA »ØÊÕ¡¢ÖÊÁ£´¿»¯µÈ·Ö×ÓÉúÎïѧʵÑ飬ÊÇͨÓÃÐÍ DNA ´¿»¯´ÅÖéÊÔ¼ÁºÐ¡£
Q10£ºÊÔ¼ÁºÐÊÒοÉÒÔ´æ·Å¶à¾Ã£¿
A£º½ö¿É¶ÌÔÝÊÒΣ¨25¡æ£©·ÅÖà 1~3 Ì죬³¤ÆÚ´æ·Å±ØÐë 2~8¡æÀ䲨£¬ÊÒξ÷Żá¼ÓËÙ´ÅÖé³Á½µÊ§Ð§¡£
A£º½ö¿É¶ÌÔÝÊÒΣ¨25¡æ£©·ÅÖà 1~3 Ì죬³¤ÆÚ´æ·Å±ØÐë 2~8¡æÀ䲨£¬ÊÒξ÷Żá¼ÓËÙ´ÅÖé³Á½µÊ§Ð§¡£
Q11£ºÍ¬Åú´Î´ÅÖé·ÖѡƬ¶Î´óС³öÏÖÆ«²îÔõô½â¾ö£¿
A£ºÐ£×¼ÒÆÒºÇ¹±£Ö¤¼ÓÑù¾«×¼£¬Ñϸñ×ñÑ˵Ã÷Êé´ÅÖéÅä±È£¬³ä·ÖÎÐÐý»ìÔÈ´ÅÖéÐüÒººóÔÙ·Ö×°ÅäÒº¡£
A£ºÐ£×¼ÒÆÒºÇ¹±£Ö¤¼ÓÑù¾«×¼£¬Ñϸñ×ñÑ˵Ã÷Êé´ÅÖéÅä±È£¬³ä·ÖÎÐÐý»ìÔÈ´ÅÖéÐüÒººóÔÙ·Ö×°ÅäÒº¡£
Q12£º´ÅÖé¿ÉÒÔÖØ¸´»ØÊÕ¡¢¶þ´ÎʹÓÃÂð£¿
A£º±¾ÊÔ¼ÁºÐ´ÅÖéΪһ´ÎÐÔʹÓúIJģ¬Îü¸½ DNA ºóÎÞ·¨ÍѸ½ÔÙÉú£¬²»ÄÜÖØ¸´ÀûÓ㬱ÜÃâÓ°ÏìºóÐø·ÖѡЧ¹û¡£
A£º±¾ÊÔ¼ÁºÐ´ÅÖéΪһ´ÎÐÔʹÓúIJģ¬Îü¸½ DNA ºóÎÞ·¨ÍѸ½ÔÙÉú£¬²»ÄÜÖØ¸´ÀûÓ㬱ÜÃâÓ°ÏìºóÐø·ÖѡЧ¹û¡£
Ïà¹Ø²úÆ·